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ScienCellGK126人类细胞表面标记qPCR检测试剂盒
发布时间:2023-01-09   点击次数:107次

GK126 ScienCell 人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNAcDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK126 ScienCell人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK126

产品名称:GK126 ScienCell人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

 

GK126 ScienCell人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit 产品说明: 

ScienCellGeneQuery™人类细胞表面标记物qPCR阵列试剂盒(GQH-CSM)调查了一组88个细胞表面标记物。细胞表面标志物是附着在细胞膜上的蛋白质,可用作鉴定和表征特定细胞类型的标志物。它们还与跨膜运输和细胞间通讯有关。下面显示了如何对基因进行分组的简要示例:

B细胞表面标志物:CD1CCD28CD79CD80CD86CR1FCGR2AITGA3MS4A1NT5E

T 细胞表面标志物:CD4CD8CD3CD6CD40LGCTLA4FASTNFRSF4

自然杀伤(NK)细胞表面标志物:CD2CD7CD96CD244CD247IL12RB1KLRNCAM1

巨噬细胞和单核细胞表面标志物:CD33CD63CD69CD70CD74DPP4IL1R2ITGA1TNFRSF8

血小板表面标志物:ITGA2BITGB3SELP

树突状细胞表面标志物:CD1ACD40CD83CD86CD209IL3RAITGAX

粒细胞表面标志物:C5AR1CEACAM8FCER1AFCER2FCGR3A

内皮细胞表面标志物:ENGICAM2MCAMNOS3PECAM1TEKVCAM1VWF

上皮细胞表面标志物:CD1DEPCAMKRT8KRT18

平滑肌细胞表面标志物:MYH9MYH10MYOCD

成纤维细胞表面标志物:ALCAMCOL1A1COL1A2

注意:所有基因名称都遵循人类基因组组织基因命名委员会(HGNC)的guanfang符号。

GeneQuery qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(iqPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO;ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Cell Surface Markers qPCR Array Kit (GQH-CSM) surveys a panel of 88 cell surface markers. Cell surface markers are proteins attached to the cell membrane that may serve as markers for the identification and characterization of specific cell types.They have also been linked to transmembrane transport and inter-cellular communication. Brief examples of how genes may be grouped are shown below:

·         B-cell surface markers: CD1C, CD28, CD79, CD80, CD86, CR1, FCGR2A, ITGA3, MS4A1, NT5E

·         T-cell surface markers: CD4, CD8, CD3, CD6, CD40LG, CTLA4, FAS, TNFRSF4

·         Natural killer (NK) cell surface markers: CD2, CD7, CD96, CD244, CD247, IL12RB1, KLRs, NCAM1

·         Macrophage and monocyte surface markers: CD33, CD63, CD69, CD70, CD74, DPP4, IL1R2, ITGA1, TNFRSF8

·         Platelet surface markers: ITGA2B, ITGB3, SELP

·         Dendritic cell surface markers: CD1A, CD40, CD83, CD86, CD209, IL3RA, ITGAX

·         Granulocyte surface markers: C5AR1, CEACAM8, FCER1A, FCER2, FCGR3A

·         Endothelial cell surface markers: ENG, ICAM2, MCAM, NOS3, PECAM1, TEK, VCAM1, VWF

·         Epithelial cell surface markers: CD1D, EPCAM, KRT8, KRT18

·         Smooth muscle cell surface markers: MYH9, MYH10, MYOCD

·         Fibroblast surface markers: ALCAM, COL1A1, COL1A2

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

 

 

基因查询™人类细胞表面标志物qPCR阵列板布局 *8 个粗体和斜体控件)

注意:所有基因名称都遵循HGNC的guanfang符号


1

2

3

4

5

6

7

8

9

10

11

12

A

ALCAM

CD2

CD37

CD6

CD79B

COL1A1

EPCAM

HLA-DRA

ITGA2B

KRT8

NT5E

ACTB

B

C5AR1

CD209

CD38

CD63

CD80

COL1A2

FAS

ICAM2

ITGA3

MCAM

PECAM1

GAPDH

C

CD160

CD22

CD3D

CD69

CD83

CR1

FCER1A

IL12RB1

ITGAX

MS4A1

SELP

LDHA

D

CD163

CD24

CD3G

CD7

CD86

CR2

FCER2

IL1R2

ITGB3

MYH10

TEK

NONO

E

CD19

CD244

CD4

CD70

CD8A

CSF1R

FCGR1A

IL2RA

KLRB1

MYH9

TNFRSF4

PPIH

F

CD1A

CD247

CD40

CD72

CD8B

CTLA4

FCGR2A

IL3RA

KLRC1

MYOCD

TNFRSF8

GDC

G

CD1C

CD28

CD40LG

CD74

CD96

DPP4

FCGR3A

ITGA1

KLRD1

NCAM1

VCAM1

PPC

H

CD1D

CD33

CD5

CD79A

CEACAM8

ENG

HLA-A

ITGA2

KRT18

NOS3

VWF

NTC

 

提示:基因选择可能会根据新的研究和开发进行更新

 

ScienCell基因表达分析产品订购信息:

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