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ScienCellGK118人类先天淋巴细胞发育标记qPCR检测试剂盒
发布时间:2023-01-06   点击次数:66次

GK118 ScienCell 人类先天淋巴细胞发育标记qPCR检测试剂盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNAcDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK118 ScienCell人类先天淋巴细胞发育标记qPCR检测试剂盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit,产品简介:

产品品牌; ScienCell

产品货号:GK118

产品名称:GK118 ScienCell人类先天淋巴细胞发育标记qPCR检测试剂盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

 

GK118 ScienCell人类先天淋巴细胞发育标记qPCR检测试剂盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit 产品说明:

ScienCell GeneQuery™ 人类先天淋巴细胞发育标记 qPCR 阵列试剂盒 (GQH-ILC) 旨在促进参与常见淋巴祖细胞 (CLP) 分化为先天淋巴细胞 (ILC)、自然杀伤 (NK) 细胞和淋巴组织诱导 (LTi) 细胞的 24 个标记基因的基因表达谱。如何根据其功能对所包含的基因进行分组的简要示例如下所示:

CLP 标记物: MME CD34 IL7R FLT3

未成熟的NK细胞标志物:CD33PROM1CD34KITIL2RBKLRK1

成熟的NK细胞标志物:FCGR3AIL2RANCAM1B3GAT1KLRD1KLRC1NCR1 / 2 / 3

ILC祖细胞标志物:CD34KITIL7R

ILC 标记:NCAM1KLRD1ITGAEIL7RKLRB1PTPRCNCR1RORC

LTi 细胞标志物:CD4IL7RCCR6

注意:所有基因名称都遵循人类基因组组织基因命名委员会(HGNC)的guanfang符号。

GeneQuery qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(iqPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO;ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Innate Lymphoid Cell Development Markers qPCR Array Kit (GQH-ILC) is designed to facilitate gene expression profiling of 24 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into innate lymphoid cells (ILCs), natural killer (NK) cells, and lymphoid tissue inducer (LTi) cells. Brief examples of how included genes may be grouped according to their functions are shown below:

·         CLP markers: MME, CD34, IL7R, FLT3

·         Immature NK cell markers: CD33, PROM1, CD34, KIT, IL2RB, KLRK1

·         Mature NK cell markers: FCGR3A, IL2RA, NCAM1, B3GAT1, KLRD1, KLRC1, NCR1/2/3

·         ILC progenitor markers: CD34, KIT, IL7R

·         ILC markers: NCAM1, KLRD1, ITGAE, IL7R, KLRB1, PTPRC, NCR1, RORC

·         LTi cell markers: CD4, IL7R, CCR6

Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

 

基因查询™ 人类先天淋巴细胞发育标志物 qPCR 阵列板布局*8 个粗体和斜体控件)

注意:所有基因名称都遵循HGNC的guanfang符号


1

2

3

4

5

6

7

8

9

10

11

12

A

B3GAT1

IL2RB

MME

ACTB

B3GAT1

IL2RB

MME

ACTB

B3GAT1

IL2RB

MME

ACTB

B

CCR6

IL7R

NCAM1

GAPDH

CCR6

IL7R

NCAM1

GAPDH

CCR6

IL7R

NCAM1

GAPDH

C

CD33

ITGAE

NCR1

LDHA

CD33

ITGAE

NCR1

LDHA

CD33

ITGAE

NCR1

LDHA

D

CD34

KIT

NCR2

NONO

CD34

KIT

NCR2

NONO

CD34

KIT

NCR2

NONO

E

CD4

KLRB1

NCR3

PPIH

CD4

KLRB1

NCR3

PPIH

CD4

KLRB1

NCR3

PPIH

F

FCGR3A

KLRC1

PROM1

GDC

FCGR3A

KLRC1

PROM1

GDC

FCGR3A

KLRC1

PROM1

GDC

G

FLT3

KLRD1

PTPRC

PPC

FLT3

KLRD1

PTPRC

PPC

FLT3

KLRD1

PTPRC

PPC

H

IL2RA

KLRK1

RORC

NTC

IL2RA

KLRK1

RORC

NTC

IL2RA

KLRK1

RORC

NTC

 

提示:基因选择可能会根据新的研究和开发进行更新

 

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