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ScienCellGK114人类小胶质细胞极化标记qPCR检测试剂盒简介
发布时间:2023-01-05   点击次数:72次

GK114 ScienCell 人类小胶质细胞极化标记qPCR检测试剂盒,Human Microglial Polarization Markers qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNAcDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK114 ScienCell人类小胶质细胞极化标记qPCR检测试剂盒,Human Microglial Polarization Markers qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK114

产品名称:GK114 ScienCell人类小胶质细胞极化标记qPCR检测试剂盒,Human Microglial Polarization Markers qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

 

 

GK114 ScienCell人类小胶质细胞极化标记qPCR检测试剂盒,Human Microglial Polarization Markers qPCR Array Kit 产品说明:

ScienCellGeneQuery™人小胶质细胞偏振标记qPCR阵列试剂盒(GQH-MPM)旨在促进参与小胶质细胞极化的40个标记基因的基因表达谱分析,这对调节先天免疫功能具有重要意义。当小胶质细胞受到刺激时,可以观察到两种潜在的表型:促炎M1小胶质细胞和抗炎M2小胶质细胞。M1表型通过释放细胞因子和撤销急性炎症来保护组织免受入侵病原体的侵害,而M2表型的作用是恢复组织稳态并刺激组织修复。如何根据其功能对所包含的基因进行分组的简要示例如下所示:   

M1 表型标记物:TNFFCGR2AFCGR2BFCGR2CFCGR3AFCGR3BCD86IL1BNOS2IL6

M2 表型标记:MRC1IL4IL10TGFB1IL1R1SOCS3ARG1IL13SPHK1

激活/静息标志物:CD80CLEC5AHEXBHLA-DQA1HLA-DQB1HLA-DRAHLA-DRB1IRF8ITGAMMYBPTGESRUNX1TLR3TLR4

其他小胶质细胞标志物:ADGRE1AIF1CD40CD68EDAITGAMITGAXPTPRC

GeneQuery qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(iqPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO;ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Microglial Polarization Markers qPCR Array Kit (GQH-MPM) is designed to facilitate gene expression profiling of 40 marker genes involved in microglial polarization, which has important implications for regulating innate immunological functions. When microglia are stimulated, two potential phenotypes can be observed: pro-inflammatory M1 microglia and anti-inflammatory M2 microglia. The M1 phenotype acts to protect tissues against invading pathogens by releasing cytokines and revoking acute inflammation, while the M2 phenotype acts to restore tissue homeostasis and stimulate tissue repair. Brief examples of how included genes may be grouped according to their functions are shown below:     

·         M1 phenotype marker: TNF, FCGR2A, FCGR2B, FCGR2C, FCGR3A, FCGR3B, CD86, IL1B, NOS2, IL6

·         M2 phenotype marker: MRC1, IL4, IL10, TGFB1, IL1R1, SOCS3, ARG1, IL13, SPHK1

·         Activation/resting markers: CD80, CLEC5A, HEXB, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, IRF8, ITGAM, MYB, PTGES, RUNX1, TLR3, TLR4

·         Additional microglia markers: ADGRE1, AIF1, CD40, CD68, EDA, ITGAM, ITGAX, PTPRC

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

 

基因查询™人小胶质细胞偏振标志物qPCR阵列板布局*8 个粗体和斜体控件)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADGRE1

EDA

HLA-DQB1

IL6

PTPRC

β-actin

ADGRE1

EDA

HLA-DQB1

IL6

PTPRC

β-actin

B

AIF1

FCGR2A

HLA-DRA

IRF8

RUNX1

GAPDH

AIF1

FCGR2A

HLA-DRA

IRF8

RUNX1

GAPDH

C

ARG1

FCGR2B

HLA-DRB1

ITGAM

SOCS3

LDHA

ARG1

FCGR2B

HLA-DRB1

ITGAM

SOCS3

LDHA

D

CD40

FCGR2C

IL10

ITGAX

SPHK1

NONO

CD40

FCGR2C

IL10

ITGAX

SPHK1

NONO

E

CD68

FCGR3A

IL13

MRC1

TGFB1

PPIH

CD68

FCGR3A

IL13

MRC1

TGFB1

PPIH

F

CD80

FCGR3B

IL1B

MYB

TLR3

GDC

CD80

FCGR3B

IL1B

MYB

TLR3

GDC

G

CD86

HEXB

IL1R1

NOS2

TLR4

PPC

CD86

HEXB

IL1R1

NOS2

TLR4

PPC

H

CLEC5A

HLA-DQA1

IL4

PTGES

TNF

NTC

CLEC5A

HLA-DQA1

IL4

PTGES

TNF

NTC

 

提示:基因选择可能会根据新的研究和开发进行更新

 

ScienCell基因表达分析产品订购信息:

GK001   人类细胞凋亡qPCR检测试剂盒,Human Apoptosis qPCR Array Kit

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GK010   人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人类黑素细胞生物学qPCR检测试剂盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人类星型胶质细胞转录因子qPCR检测试剂盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR检测试剂盒,Human Angiogenesis qPCR Array Kit

GK018   人内皮细胞表型异质性qPCR检测试剂盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

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GK020   B细胞受体信号通路qPCR检测试剂盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

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GK022   人内吞作用qPCR检测试剂盒,Human Endocytosis qPCR Array Kit

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GK024   人体剪切应力和机械传导qPCR检测试剂盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

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GK026   人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit

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GK029   Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纤维化qPCR检测试剂盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人体缺氧反应qPCR检测试剂盒,Human Hypoxia Response qPCR Array Kit

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GK073   人类小胶质细胞Sensome qPCR检测试剂盒,Human Microglial Sensome qPCR Array Kit

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