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ScienCell GK106人类焦点粘附复合物qPCR检测试剂盒简介
发布时间:2023-01-04   点击次数:90次

GK106 ScienCell 人类焦点粘附复合物qPCR检测试剂盒,Human Focal Adhesion Complexes qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNAcDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK106 ScienCell人类焦点粘附复合物qPCR检测试剂盒,Human Focal Adhesion Complexes qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK106

产品名称:GK106 ScienCell人类焦点粘附复合物qPCR检测试剂盒,Human Focal Adhesion Complexes qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

 

GK106 ScienCell人类焦点粘附复合物qPCR检测试剂盒,Human Focal Adhesion Complexes qPCR Array Kit 产品说明: 

ScienCell GeneQuery™ 人类粘连复合物 qPCR 阵列试剂盒 (GQH-FAC) 分析了参与调节和形成粘连的 88 个关键基因。 局灶性粘连是细胞与其细胞外基质(ECM)环境相互作用的大分子复合物。 它们参与各种过程,如解释机械力,调节细胞粘附和运动,以及促进细胞内信号转导。 整合素和粘连激酶(PTK2)是粘连的主要调节元件。 如何对所包含的基因进行分类的简要示例如下所示:

AK 信令: CCND1 FYN PTK2 RHOA SRC

细胞骨架元素:ACTBACTN1ACTN3FLNAVCL

ECM 组件: COL1A2 COL2A1 COL4A2 FN1 LAMC3

ECM 绑定元素:ITGA1ITGA5ITGAMITGB1ITGB3

信号转导基因:ARHGAP5ELK1HGFJUNKDR

GeneQuery qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(iqPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO;ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

 ScienCell's GeneQuery™ Human Focal Adhesion Complexes qPCR Array Kit (GQH-FAC) profiles 88 key genes involved in the regulation and formation of focal adhesions.  Focal adhesions are large macromolecular complexes by which the cell interacts with its extracellular matrix (ECM) environment.  They are involved in such diverse processes as interpreting mechanical force, regulating cell adhesion and motility, and contributing to intracellular signaling transduction.  Integrins and focal adhesion kinase (PTK2) are the main regulatory elements of focal adhesions.  Brief examples of how included genes may be categorized are shown below:

·         FAK signaling: CCND1, FYN, PTK2, RHOA, SRC

·         Cytoskeletal elements: ACTB, ACTN1, ACTN3, FLNA, VCL

·         ECM components: COL1A2, COL2A1, COL4A2, FN1, LAMC3

·         ECM binding elements: ITGA1, ITGA5, ITGAM, ITGB1, ITGB3

·         Signaling transduction genes: ARHGAP5, ELK1, HGF, JUN, KDR

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

 

GeneQuery™ 人粘附复合物 qPCR 阵列板布局*8 个粗体和斜体对照)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTB

BRAF

COL4A2

FYN

ITGA3

ITGAL

ITGB5

MAP2K1

PAK2

PXN

SHC1

β-actin

B

ACTN1

CAV1

CRK

GRB2

ITGA4

ITGAM

ITGB6

MAPK1

PAK4

RAC1

SHC3

GAPDH

C

ACTN3

CAV2

DOCK1

HGF

ITGA5

ITGAV

ITGB7

MAPK10

PDGFRA

RAF1

SOS1

LDHA

D

AKT1

CAV3

EGFR

HRAS

ITGA6

ITGAX

JUN

MAPK8

PDGFRB

RAP1A

SRC

NONO

E

AKT2

CCND1

ELK1

ILK

ITGA7

ITGB1

KDR

MAPK9

PDPK1

RAPGEF1

TLN1

PPIH

F

AKT3

CDC42

FLNA

ITGA1

ITGA8

ITGB2

LAMA1

MET

PIK3R1

RHOA

VAV3

GDC

G

ARHGAP5

COL1A2

FLT1

ITGA2

ITGAD

ITGB3

LAMB1

MYLK2

PIK3R2

ROCK1

VCL

PPC

H

BCAR1

COL2A1

FN1

ITGA2B

ITGAE

ITGB4

LAMC3

PAK1

PTK2

ROCK2

VTN

NTC

 

提示:基因选择可能会根据新的研究和开发进行更新

 

ScienCell基因表达分析产品订购信息:

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