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ScienCellGK095人间充质至上皮细胞转化qPCR检测的特点
发布时间:2022-12-30   点击次数:83次

GK095 ScienCell 人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNAcDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的特约经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK095 ScienCell人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK095

产品名称:GK095 ScienCell人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

北京裕恒丰科技有限公司是ScienCell中国区总代理,提供各种基因表达分析相关品,ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK095 ScienCell人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit 产品说明:

ScienCell GeneQuery™ 人间充质到上皮转化 qPCR 阵列试剂盒 (GQH-MET) 旨在促进参与可逆间充质到上皮转化 (MET) 的 88 个关键基因的基因表达谱分析。MET和反向过程,上皮到间充质转化(EMT),参与胚胎发生,癌症转移和干细胞重编程。下面显示了如何对基因进行分组的简要示例:    

EMT标记: CDH1 CDH2 VIM FN1 CDH11 SDC1 S100A4 ACTA2 CTNNB1 LEF1 ETS1 FOXC2 GSC PDGFD

EMT-MET过渡:PAX2WT1BMP7TWIST1PRRX1

增殖和形态发生控制:AKT1BMP1BMP7EGFRFGFBP1IGFBP4JAG1PDGFRBSNAI1SOX10VCANWNT5AWNT11ZEB1

分化控制: BMP2 COL3A1 ERBB3 F11R FZD7 GSC JAG1 KRT14, 节点, SMAD2 SOX10 WNT5B

调节 MET 的转录因子:CTNNB1ESR1FOXC2NOTCH1SMAD2SNAI1/2/3STAT3TCF3TCF4TWIST1ZEB2

MET中涉及的信号通路

雌激素受体:CAV2ESR1KRT19TGFB3

GPCR AKT1FZD7 GNG11 RAC1 RGS2

PI3K/AKT AKT1 AKT2 PIK3CA PIK3CD PIK3CG PIK3R1 PIK3R3 PIK3R5

GeneQuery qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(iqPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO;ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Mesenchymal To Epithelial Transition qPCR Array Kit (GQH-MET) is designed to facilitate gene expression profiling of 88 key genes involved in reversible mesenchymal to epithelial transition (MET). MET and the reverse process, epithelial to mesenchymal transition (EMT), are involved in embryogenesis, cancer metastasis, and stem cell reprogramming. Brief examples of how genes may be grouped are shown below:    

·         EMT markers: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD

·         EMT-MET transition: PAX2, WT1, BMP7, TWIST1, PRRX1

·         Proliferation and morphogenesis control: AKT1, BMP1, BMP7, EGFR, FGFBP1, IGFBP4, JAG1, PDGFRB, SNAI1, SOX10, VCAN, WNT5A, WNT11, ZEB1

·         Differentiation control: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, NODAL, SMAD2, SOX10, WNT5B

·         Transcription factors regulating MET: CTNNB1, ESR1, FOXC2, NOTCH1, SMAD2, SNAI1/2/3, STAT3, TCF3, TCF4, TWIST1, ZEB2

·         Signaling pathways involved in MET

o    Estrogen receptor: CAV2, ESR1, KRT19, TGFB3

o    GPCR: AKT1, FZD7, GNG11, RAC1, RGS2

o    PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人间充质到上皮过渡 qPCR 阵列板布局*8 个粗体和斜体控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CAV2

COL3A1

F11R

IGFBP4

MUC1

PIK3CG

RGS2

SNAI3

TGFB2

VCAN

β-actin

B

AKT1

CDH1

COL5A2

FGFBP1

ILK

NODAL

PIK3R1

RPS27A

SOX10

TGFB3

VIM

GAPDH

C

AKT2

CDH11

CTNNB1

FN1

JAG1

NOTCH1

PIK3R3

S100A4

SPINT2

TGFBR1

WNT11

LDHA

D

ARHGEF18

CDH2

DSP

FOXC2

KRT14

PAX2

PIK3R5

SDC1

STAT3

TGFBR2

WNT5A

NONO

E

AXL

CDKN2A

EGFR

FZD7

KRT19

PDGFD

PRKCZ

SMAD2

STK11

TIMP1

WNT5B

PPIH

F

BMP1

CDKN2B

ERBB3

GNG11

LEF1

PDGFRB

PRRX1

SMURF1

TCF3

TRIM28

WT1

GDC

G

BMP2

CLDN4

ESR1

GSC

MMP2

PIK3CA

RAB25

SNAI1

TCF4

TWIST1

ZEB1

PPC

H

BMP7

CLDN7

ETS1

HNRNPAB

MST1R

PIK3CD

RAC1

SNAI2

TGFB1

UBA52

ZEB2

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

GK001   人类细胞凋亡qPCR检测试剂盒,Human Apoptosis qPCR Array Kit

GK002   人类多能干细胞qPCR检测试剂盒,Human Pluripotent Stem Cell qPCR Array Kit

GK003   人类细胞周期qPCR检测试剂盒,Human Cell Cycle qPCR Array Kit

GK005   人上皮细胞生物学qPCR检测试剂盒,Human Epithelial Cell Biology qPCR Array Kit

GK006   人类神经发育和再生qPCR检测试剂盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人类神经可塑性qPCR检测试剂盒,Human Neural Plasticity qPCR Array Kit

GK008   人类神经传递和膜转运qPCR检测试剂盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

GK009   人类星型胶质细胞生物学qPCR检测试剂盒,Human Astrocyte Cell Biology qPCR Array Kit

GK010   人类黑素细胞发育和色素沉着qPCR检测试剂盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人类黑素细胞生物学qPCR检测试剂盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人类星型胶质细胞转录因子qPCR检测试剂盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR检测试剂盒,Human Angiogenesis qPCR Array Kit

GK018   人内皮细胞表型异质性qPCR检测试剂盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人内皮细胞生物学qPCR检测试剂盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B细胞受体信号通路qPCR检测试剂盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人类T细胞受体信号通路qPCR检测试剂盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人内吞作用qPCR检测试剂盒,Human Endocytosis qPCR Array Kit

GK023   人皮内细胞分化qPCR检测试剂盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人体剪切应力和机械传导qPCR检测试剂盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人类Notch信号通路qPCR检测试剂盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人类Wnt信号通路qPCR检测试剂盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人类非经典Wnt信号通路qPCR检测试剂盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人类Wnt信号靶基因qPCR检测试剂盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纤维化qPCR检测试剂盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人体缺氧反应qPCR检测试剂盒,Human Hypoxia Response qPCR Array Kit

GK036   人类HIF1信号响应qPCR检测试剂盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit

GK038   人类吞噬作用qPCR检测试剂盒,Human Phagocytosis qPCR Array Kit

GK039   人类先天免疫反应qPCR检测试剂盒,Human Innate Immune Response qPCR Array Kit

GK040   人成纤维细胞生长因子信号通路qPCR检测试剂盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人体皮肤伤口愈合qPCR检测试剂盒,Human Skin Wound Healing qPCR Array Kit

GK056   人体止血qPCR检测试剂盒,Human Hemostasis qPCR Array Kit

GK060   人类昼夜节律qPCR检测试剂盒,Human Circadian Rhythm qPCR Array Kit

GK061   人类DNA损伤感应qPCR检测试剂盒,Human DNA Damage Sensing qPCR Array Kit

GK062   人类细胞生长和分裂qPCR检测试剂盒,Human Cell Growth and Division qPCR Array Kit

GK063   人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

GK069   人类小胶质细胞生物学qPCR检测试剂盒,Human Microglia Cell Biology qPCR Array Kit

GK072   人类适应性免疫反应qPCR检测试剂盒,Human Adaptive Immune Response qPCR Array Kit

GK073   人类小胶质细胞Sensome qPCR检测试剂盒,Human Microglial Sensome qPCR Array Kit

GK074   人角质细胞生物学qPCR检测试剂盒,Human Keratocyte Biology qPCR Array

GK078   人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR检测试剂盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人类周细胞生物学qPCR检测试剂盒,Human Pericyte Biology qPCR Array Kit

GK084   人体轴突导向qPCR检测试剂盒,Human Axon Guidance qPCR Array kit

GK087   人类染色质组织和重塑qPCR检测试剂盒,Human Chromatin Organization and Remodeling qPCR Array Kit

GK088   VEGF信号通路qPCR检测试剂盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089   人类Ephrin信号qPCR检测试剂盒,Human Ephrin Signaling qPCR Array Kit

GK090   人体细胞连接qPCR检测试剂盒,Human Cell Junctions qPCR Array Kit

GK091   人肝星状细胞生物学qPCR检测试剂盒,Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092   人胰腺星状细胞生物学qPCR检测试剂盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array KitHuman Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR ArrayHuman Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095   人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096   人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit

GK097   人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit

GK098   人类心脏肌细胞生物学qPCR检测试剂盒,Human Cardiac Myocyte Biology qPCR Array Kit

GK099   人类骨骼肌细胞生物学qPCR检测试剂盒,Human Skeletal Muscle Cell Biology qPCR Array Kit

GK100   人角质形成细胞生物学qPCR检测试剂盒,Human Keratinocyte Cell Biology qPCR Array Kit

GK101   人类Synoviocyte细胞生物学qPCR检测试剂盒,Human Synoviocyte Cell Biology qPCR Array Kit

GK102   人类滋养层细胞生物学qPCR检测试剂盒,Human Trophoblast Cell Biology qPCR Array Kit

GK103   人前脂肪细胞生物学qPCR检测试剂盒,Human Preadipocyte Cell Biology qPCR Array Kit

GK104   人类细胞骨架重塑qPCR检测试剂盒,Human Cytoskeletal Remodeling qPCR Array Kit

GK105   人类ECM降解qPCR检测试剂盒,Human ECM Degradation qPCR Array Kit

GK106   人类焦点粘附复合物qPCR检测试剂盒,Human Focal Adhesion Complexes qPCR Array Kit

GK107   人内皮素信号qPCR检测试剂盒,Human Endothelin Signaling qPCR Array Kit

GK111   人凝集素调节免疫应答qPCR检测试剂盒,Human Lectin Regulation in Immune Response qPCR Array Kit

GK112   人类神经分化标记qPCR检测试剂盒,Human Neural Differentiation Markers qPCR Array Kit

GK113   人类神经干细胞标记qPCR检测试剂盒,Human Neural Stem Cell Markers qPCR Array Kit

GK114   人类小胶质细胞极化标记qPCR检测试剂盒,Human Microglial Polarization Markers qPCR Array Kit

GK115   人类骨髓谱系标记qPCR检测试剂盒,Human Myeloid Lineage Markers qPCR Array Kit

GK116   B细胞和T细胞发育标记qPCR检测试剂盒,Human B and T Cells Development Markers qPCR Array Kit

GK117   人胸腺细胞发育标记qPCR检测试剂盒,Human Thymocyte Development Markers qPCR Array Kit

GK118   人类先天淋巴细胞发育标记qPCR检测试剂盒,Human Innate Lymphoid Cell Development Markers qPCR Array Kit

GK119   人类神经祖细胞标记qPCR检测试剂盒,Human Neural Progenitor Markers qPCR Array Kit

GK121   PD-1/PD-L1检查点途径qPCR检测试剂盒,GeneQuery Human PD-1/PD-L1 Checkpoint Pathway qPCR Array Kit

GK123   人类生长因子qPCR检测试剂盒,Human Growth Factors qPCR Array Kit

GK124   人类转录因子qPCR检测试剂盒,Human Transcription Factors qPCR Array Kit

GK125   人干细胞转录因子qPCR检测试剂盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit

GK127   人脑发育标记物qPCR检测试剂盒,Human Brain Development Markers qPCR Array Kit



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