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ScienCellGK062人类细胞生长和分裂qPCR检测试剂盒
发布时间:2022-12-22   点击次数:79次

GK062 ScienCell 人类细胞生长和分裂qPCR检测试剂盒,Human Cell Growth and Division qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNAcDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK062 ScienCell人类细胞生长和分裂qPCR检测试剂盒,Human Cell Growth and Division qPCR Array Kit,产品简介:

产品品牌:  ScienCell

产品货号:GK062

产品名称:GK062 ScienCell人类细胞生长和分裂qPCR检测试剂盒,Human Cell Growth and Division qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well


GK062 ScienCell人类细胞生长和分裂qPCR检测试剂盒,Human Cell Growth and Division qPCR Array Kit 产品说明:

ScienCell GeneQuery™ 人类细胞生长和分裂 qPCR 阵列试剂盒 (GQH-CGD) 旨在促进参与细胞生长和分裂的 88 个关键基因的基因表达谱分析。严格控制这些过程对于确保遗传信息从亲本细胞正确传递到子细胞以及严格调节细胞生长至关重要。这种控制失败会导致突变和/或肿瘤发生。下面显示了如何对所包含的基因进行分组的简要示例:  

细胞周期调节:ATMCCNA2CCNB1CCNE2CDC20CDC25CCDK1CDK2CHEK1NBNRAD1RAD17

有丝分裂:APC/C 复合体、CDC 基因、BUB1BUB1BEGFEREGKNTC1NPM2MAD2L1MAD2L2PKMYT1TGFATGFB1TTK

减数分裂:APC/C 复合体、CDC 基因、RAD51RBX1SKP1SMC1ASMC3

主轴检查点: AURKA BIRC5 BUB1 CDC16 CDC27 DCTN1 KNTC1 PAFAH1B1 SMC1A SMC3 STMN1 TTK

GeneQuery qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(iqPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO;ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery Human Cell Growth and Division qPCR Array Kit (GQH-CGD) is designed to facilitate gene expression profiling of 88 key genes involved in cell growth and division. Tight control of these processes is essential to ensure that the hereditary information is correctly passed on from parent cells to daughter cells, and that cell growth is strictly regulated. Failure of such control gives rise to mutations and/or tumorigenesis. Brief examples of how included genes may be grouped are shown below:    

·         Cell cycle regulation: ATM, CCNA2, CCNB1, CCNE2, CDC20, CDC25C, CDK1, CDK2, CHEK1, NBN, RAD1, RAD17

·         Mitosis: APC/C complex, CDC genes, BUB1, BUB1B, EGF, EREG, KNTC1, NPM2, MAD2L1, MAD2L2, PKMYT1, TGFA, TGFB1, TTK

·         Meiosis: APC/C complex, CDC genes, RAD51, RBX1, SKP1, SMC1A, SMC3

·         Spindle checkpoint: AURKA, BIRC5, BUB1, CDC16, CDC27, DCTN1, KNTC1, PAFAH1B1, SMC1A, SMC3, STMN1, TTK

GeneQuery qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人类细胞生长和分裂qPCR阵列板布局*8 个粗体和斜体控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ADCY1

ANAPC2

BUB1

CCNE2

CDC27

EGF

KNTC1

NBN

PPP3CA

RAD17

TARDBP

β-actin

B

ADCY2

ANAPC4

BUB1B

CD28

CDK1

EREG

MAD2L1

NPM2

PPP3R1

RAD51

TGFA

GAPDH

C

ADCY3

ANAPC5

CALM1

CDC16

CDK2

ESPL1

MAD2L2

PAFAH1B1

PRKACA

RBX1

TGFB1

LDHA

D

ADCY8

ANAPC7

CAMK2A

CDC20

CDKN2B

FBXW11

MAP2K1

PBRM1

PRKACB

RPS6KA1

TTK

NONO

E

ANAPC1

ATM

CAMK2G

CDC23

CHEK1

IGF1

MAPK1

PKMYT1

PRKACG

SKP1

TTN

PPIH

F

ANAPC10

AURKA

CCNA2

CDC25B

CHP2

IGF1R

MAPK12

PLK1

PRKX

SMC1A

YWHAB

GDC

G

ANAPC11

BIRC5

CCNB1

CDC25C

CUL1

ITPR1

MAPK3

PPP1CA

PTTG1

SMC3

YWHAE

PPC

H

ANAPC13

BTRC

CCNB2

CDC26

DCTN1

ITPR2

MRE11A

PPP2CA

RAD1

STMN1

YWHAG

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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GK013   人类星型胶质细胞转录因子qPCR检测试剂盒,Human Astrocyte Transcription Factors qPCR Array Kit

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GK020   B细胞受体信号通路qPCR检测试剂盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

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GK022   人内吞作用qPCR检测试剂盒,Human Endocytosis qPCR Array Kit

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GK027   人类非经典Wnt信号通路qPCR检测试剂盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人类Wnt信号靶基因qPCR检测试剂盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纤维化qPCR检测试剂盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人体缺氧反应qPCR检测试剂盒,Human Hypoxia Response qPCR Array Kit

GK036   人类HIF1信号响应qPCR检测试剂盒,Human HIF1 Signaling Response qPCR Array Kit

GK037   人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit

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GK069   人类小胶质细胞生物学qPCR检测试剂盒,Human Microglia Cell Biology qPCR Array Kit

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GK073   人类小胶质细胞Sensome qPCR检测试剂盒,Human Microglial Sensome qPCR Array Kit

GK074   人角质细胞生物学qPCR检测试剂盒,Human Keratocyte Biology qPCR Array

GK078   人类神经细胞生物学qPCR检测试剂盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人类软骨细胞生物学qPCR检测试剂盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR检测试剂盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人类周细胞生物学qPCR检测试剂盒,Human Pericyte Biology qPCR Array Kit

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GK088   VEGF信号通路qPCR检测试剂盒,Human VEGF Signaling Pathway qPCR Array Kit

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GK093   Human Renal Mesangial Cell Biology qPCR Array KitHuman Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR ArrayHuman Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095   人间充质至上皮细胞转化qPCR检测试剂盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096   人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit

GK097   人类平滑肌细胞生物学qPCR检测试剂盒,Human Smooth Muscle Cell Biology qPCR Array Kit

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GK126   人类细胞表面标记qPCR检测试剂盒,Human Cell Surface Markers qPCR Array Kit

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