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ScienCellGK037人巨噬细胞细胞生物学qPCR检测试剂盒
更新时间:2023-01-03   点击次数:345次

GK037 ScienCell 人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNAcDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京诺博莱德作为ScienCell品牌的经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK037 ScienCell人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK037

产品名称:GK037 ScienCell人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well


GK037 ScienCell人巨噬细胞细胞生物学qPCR检测试剂盒,Human Macrophage Cell Biology qPCR Array Kit 产品说明:

ScienCellGeneQuery™人类巨噬细胞生物学qPCR阵列试剂盒(GQH-MAC)旨在促进参与巨噬细胞活化和功能的88个关键基因的基因表达谱分析。 巨噬细胞是人体免疫反应系统的重要组成部分,被激活用于各种目的。 如何根据激活或功能对所包含的基因进行分组的简要示例如下所示:

炎症反应:F2RPTAFRNPY1RPTGER1TACR1

趋化性: CXCL8 MMP2 MMP9 PTGS2 FPR1

经典活化:CCL15IL12ANOS2TNFIFNG

调节激活: IL10 CD80 CD86 SPHK1 CCL1

伤口愈合激活:ARG1LPAR1SIPR1IL27RAMRC1

GeneQuery qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(iqPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO;ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Macrophage Cell Biology qPCR Array Kit (GQH-MAC) is designed to facilitate gene expression profiling of 88 key genes involved in macrophage activation and function.  Macrophages are an important component of the human immune response system and are activated for various purposes.  Brief examples of how included genes may be grouped according to activation or function are shown below:

·         Inflammatory response: F2R, PTAFR, NPY1R, PTGER1, TACR1

·         Chemotaxis: CXCL8, MMP2, MMP9, PTGS2, FPR1

·         Classical activation: CCL15, IL12A, NOS2, TNF, IFNG

·         Regulatory activation: IL10, CD80, CD86, SPHK1, CCL1

·         Wound healing activation: ARG1, LPAR1, SIPR1, IL27RA, MRC1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人巨噬细胞生物学 qPCR 阵列板布局*8 个粗体和斜体对照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ADGRE1

CCL15

CD80

CXCL8

F2RL2

IFNB1

IL27RA

LTB4R2

NOX1

PTGER4

SOCS1

β-actin

B

ADGRE2

CCL17

CD86

CXCL9

FCER2

IFNG

IL4R

MAPK1

NPY1R

PTGES2

SPHK1

GAPDH

C

ADGRE5

CCL18

CD93

CXCR4

FCGR1A

IGF1

IL6

MARCO

NPY2R

PTGS2

SRA1

LDHA

D

ARG1

CCL20

CIITA

EDNRA

FCGR1B

IL10

ITGAM

MMP2

PIK3R1

SIGLEC1

STAB1

NONO

E

ARG2

CCL22

CLEC4A

EDNRB

FCGR3A

IL12A

LAMP1

MMP9

PTAFR

SIPR1

TACR1

PPIH

F

C5AR1

CCR5

CLEC7A

F13A1

FPR1

IL12B

LPAR1

MRC1

PTGER1

SIPR2

TGM2

GDC

G

C5AR2

CD163

CXCL10

F2R

FPR3

IL1A

LPAR2

NFKB1

PTGER2

SIPR5

TNF

PPC

H

CCL1

CD68

CXCL11

F2RL1

IDO1

IL23A

LTB4R

NOS2

PTGER3

SLAMF8

TNFRSF18

NTC\

提示:基因选择可能会根据新的研究和开发进行更新

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