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AnaSpec AS-72263 SensoLyte® 简介
发布时间:2021-08-27   点击次数:423次

AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*1 kit

AnaSpec ( 美国 )

AnaSpec公司成立于1993年,是一家为全球生命科学研究,提供综合蛋白质组学和基因组学解决方案的lingxian供应商。
公司产品主要分三大部分:提供专业多肽,检测试剂,抗体检测试剂盒;适合基础研究的高通量筛选和新药物研发相关试剂;多肽合成,抗体生产,寡核苷酸的合成等定制服务。

我们北京诺博莱德科技有限公司作为AnaSpec公司的经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*1 kit,产品简介:

产品品牌:AnaSpec

产品名称:SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*

英文名称:SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*

产品货号:AS-72263

产品规格:1 kit

AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*1 kit,产品说明:

SensoLyte®520 SARS-CoV-2类木瓜蛋白酶/去泛素酶活性检测试剂盒采用荧光肽底物检测去泛素化活性。当活性木瓜蛋白酶样蛋白酶切割底物时,在激发/发射=490 nm/520 nm时,会导致亮绿色荧光增加。该检测方法可检测样品中低至7.8 ng/mL的活性木瓜蛋白酶。该试剂盒可用于检测纯化酶制剂、生物样品中的酶活性,也可用于化合物筛选。

SARS-Cov-2木瓜蛋白酶样蛋白酶(PLpro)编码为非结构蛋白3nsp3),参与病毒多蛋白的切割。这种酶水解泛素链,泛素链在炎症过程中起重要作用,并从蛋白质中去除干扰素刺激的基因15ISG15)修饰以逆转抗病毒反应。PLpro作为SARS-CoV-2复制和感染宿主过程中的一种必需酶,已成为冠状病毒抑制剂开发的一个有吸引力的药物靶点。

The SensoLyte® 520 SARS-CoV-2 Papain-like Protease/ Deubiquitinase Activity Assay Kit employs a fluorescent peptide substrate for the detection of deubiquitnation activity. When active Papain-like Protease cleaves the substrate, it results in an increase of bright green fluorescence at excitation/emission = 490 nm/520 nm. This assay can detect as low as 7.8 ng/mL active Papain-like Protease in the sample. This kit can be used to detect enzyme activity in purified enzyme preparations, biological samples and can also be applied for the compound screening.

SARS-Cov-2 Papain-like Protease (PLpro) is encoded as non-structural protein 3 (nsp3) and it is involved in the cleavage of the viral polyprotein. This enzyme hydrolyzes ubiquitin chains which are important in the inflammation process and also removes interferon-stimulated gene 15 (ISG15) modifications from proteins to reverse antiviral responses. As an essential enzyme in the process of SARS-CoV-2 replication and infection of the host, PLpro has been an attractive drug target for the coronavirus inhibitor development.

Kits components 产品组件:

Component A: Papain-like Protease Deubiquitination substrate Ex/Em=490 nm/520 nm upon cleavage; 400µM, 50µL Component B: Rh110 fluorescence reference standard, Ex/Em=490 nm/520 nm; 400µM, 15µL Component C: Recombinant SARS-CoV-2 Papain-like protease; 0.1mg/mL, 10µL Component D: 2X Assay Buffer, 25mL Component E: Inhibitor (GRL0617); 10µM, 10µL

组分A:木瓜蛋白酶类去泛素化底物Ex/Em=490nm/520nm400µM50µL组分B:Rh110荧光对照品,Ex/Em=490 nm/520 nm400µM15µL组分C:重组SARS-CoV-2木瓜蛋白酶;0.1mg/mL10µL组分D:2X分析缓冲液,25mL组分E:抑制剂(GRL0617);10µM10µL

AnaSpec品牌部分产品货号:

品牌

货号

名称

规格

AnaSpec

AS-72262

SensoLyte®   SARS-CoV-2 3CL Protease Activity Assay Kit *Fluorimetric*

1 kit

AnaSpec

AS-72263

SensoLyte® SARS-CoV-2 Papain-like Protease   (PLpro) Activity Assay Kit

1 kit

AnaSpec

AS-55551

SensoLyte®   Anti-Human β-Amyloid   (1-40) Quantitative ELISA Kit *Colorimetric*

1 kit

AnaSpec

AS-55552

SensoLyte® Anti-Human β-Amyloid (1-42)   Quantitative ELISA Kit *Colorimetric*

1 kit

AnaSpec

AS-72246

AnaTag Europium Protein   Labeling Kit

1 kit

AnaSpec

AS-72247

AnaTag Protein Labeling Kit   for TR-FRET - 1 kit

1 kit

AnaSpec

AS-71120

SensoLyte® AMC   Caspase Profiling Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-71121

SensoLyte® AMC Caspase   Substrate Sampler Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-71124

SensoLyte® Green   Protease Assay Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-71126

SensoLyte® 490 HCV   Protease Assay Kit Fluorimetric 500 assays - 1 kit

1 kit

AnaSpec

AS-71127

SensoLyte® 490 HIV   Protease Assay Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-71128

SensoLyte® 490   MMP-1 Assay Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-71129

SensoLyte® 490   MMP-2 Assay Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-72040

SensoLyte® 520   Renin Assay Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-72122

SensoLyte® Luminescent Alkaline Phosphatase   Assay Kit Luminometric - 1 kit

1 kit

AnaSpec

AS-72129

SensoLyte® 520 Thrombin   Activity Assay Kit Fluorimetric - 1 kit

1 kit

AnaSpec

AS-20276

Beta-Amyloid (1-42)

1 mg

AnaSpec

AS-20276-25

Beta-Amyloid (1-42)

25 mg

AnaSpec

AS-20276-5

Beta-Amyloid (1-42)

5 mg

AnaSpec

AS-20291

Threonine Phosphopeptide,   PKC Substrate 4, phosphorylated - 1 mg

1 mg

AnaSpec

AS-20292

Insulin Receptor (1142-1153),   pTyr1146 - 1 mg

1 mg

AnaSpec

AS-21158

HA peptide

5 mg

AnaSpec

AS-24467

Exendin (9-39)

0.5 mg

AnaSpec

AS-24468

Exendin (9-39)

1 mg

AnaSpec

AS-64129-05

Beta-Amyloid (1-42)     HFIP

0.5 mg

AnaSpec

AS-64129-1

Beta-Amyloid (1-42)     HFIP

1 mg

AnaSpec

AS-72215

AggreSure ß-Amyloid   (1-40), human - 0.25 mg

0.25 mg NET peptide

AnaSpec

AS-72216

AggreSure ß-Amyloid   (1-42), human - 0.25 mg

0.25 mg NET peptide

AnaSpec

AS-72049

AnaTag HiLyte Fluor 647 Protein Labeling Kit - 1 kit

1 kit

AnaSpec

AS-72050

AnaTag HiLyte Fluor 647 Microscale Protein Labeling Kit - 1 kit

1 kit

AnaSpec

AS-72111

AnaTag APC Labeling Kit - 1   kit

1 kit

AnaSpec

AS-72113

AnaTag R - PE Labeling Kit -   1 kit

1 kit

AnaSpec

AS-55158-100

Recombinant human   MOG (1-125)

100 µg

AnaSpec

AS-55158-1000

Recombinant human   MOG (1-125)

1000 µg

AnaSpec

AS-55158-500

Recombinant human   MOG (1-125)

500 µg

AnaSpec

AS-55525-1

MMP-12 (Catalytic   Domain), human recombinant

1 µg

AnaSpec

AS-55525-10

MMP-12 (Catalytic   Domain), human recombinant

10 µg

AnaSpec

AS-55525-50

MMP-12 (Catalytic   Domain), human recombinant

50 µg

AnaSpec

AS-72237

Protein A-HiLyte Fluor 647 Conjugate -   1 mg

1 mg

AnaSpec

AS-72239

Protein A-HiLyte Fluor 750 Conjugate -   1 mg

1 mg

AnaSpec

AS-72237

Protein A-HiLyte Fluor 647 Conjugate -   1 mg

1 mg

AnaSpec

AS-72239

Protein A-HiLyte Fluor 750 Conjugate -   1 mg

1 mg

如需其他产品请联系本店客服。






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